Part:BBa_K1624008

pCoof->RBS

pCoof promoter followed by ribosome binding site. May be placed in front of genes to regulate expression in response to CO presence.

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Usage and Biology

We placed a reporter gene, sfGFP (BBa_K1624004), downstream of the pCoof-RBS in the pCDFDuet-1 plasmid. We transformed this plasmid along with another plasmid containing a constitutively produced transcription activator CooA into BL21(DE3) cells. We used cells without the modified plasmid as our negative control, and cells containing plasmids with T7-regulated sfGFP as our positive control. We tested the constructs at 0, 50, 100, 200 and 500 uM CORM-2 (carbon monoxide releasing molecule). pCoof regulated sfGFP did not show a significant correlation between gene expression and CO presence. However, other factors (listed in link below) may have influenced our results. More details here: http://2015.igem.org/Team:LASATX/Results.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]